vglut2, rabbit polyclonal antibody Search Results


vglut2  (OriGene)
93
OriGene vglut2
Fig. 3. Transduced neuronal cell types. Spinal sections were processed for immunoreactivity to NeuN, <t>VGLUT2,</t> GAD65, and ChAT to identify the neuronal cell types associated with the neurons transduced by ChR2-mCherry or Chronos-GFP adeno- associated viruses (AAV). White arrows highlight some of the positively stained GAD65, VGLUT2, and ChAT puncta that colocalized with transduced neurons. A–D: a representative spinal section transduced by ChR2-mCherry AAV (A; red) and stained for NeuN (B; blue), and VGLUT2 (C; green). D: merged view, indicating that the ChR2-mCherry AAV trans- duced neurons that are associated with glutamatergic neu- rotransmission. E–H: a second representative spinal section transduced by ChR2-mCherry AAV (E; red) stained for NeuN (F), and GAD65 (G). H: merged view showing that the ChR2- mCherry AAV transduced neurons associated with GABAergic transmission. I–L: a representative spinal section transduced by Chronos-GFP AAV (I; green) and stained for NeuN (J; blue), and VGLUT2 (K; red). The merged view is depicted in L indicating that the Chronos-GFP AAV transduced neurons associated with glutamatergic neurotransmission. M–P: a sec- ond representative spinal section transduced by Chronos-GFP AAV (M) and stained for NeuN (N), and GAD65 (O). The merged view is shown in P, indicating that the Chronos-GFP AAV also transduced neurons associated with GABAergic transmission. Q–T: a representative spinal section transduced by Chronos-GFP AAV (Q; green), NeuN (R; blue), and ChAT (S; red). The merged view is shown in T indicating that the Chronos vector also labels a small percentage of neurons associated with cholinergic transmission. Scale bar 20 m.
Vglut2, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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polyclonal rabbit vesicular glutamate transporter 2 (vglut2  (GeneTex)
90
GeneTex polyclonal rabbit vesicular glutamate transporter 2 (vglut2
Fig. 3. Transduced neuronal cell types. Spinal sections were processed for immunoreactivity to NeuN, <t>VGLUT2,</t> GAD65, and ChAT to identify the neuronal cell types associated with the neurons transduced by ChR2-mCherry or Chronos-GFP adeno- associated viruses (AAV). White arrows highlight some of the positively stained GAD65, VGLUT2, and ChAT puncta that colocalized with transduced neurons. A–D: a representative spinal section transduced by ChR2-mCherry AAV (A; red) and stained for NeuN (B; blue), and VGLUT2 (C; green). D: merged view, indicating that the ChR2-mCherry AAV trans- duced neurons that are associated with glutamatergic neu- rotransmission. E–H: a second representative spinal section transduced by ChR2-mCherry AAV (E; red) stained for NeuN (F), and GAD65 (G). H: merged view showing that the ChR2- mCherry AAV transduced neurons associated with GABAergic transmission. I–L: a representative spinal section transduced by Chronos-GFP AAV (I; green) and stained for NeuN (J; blue), and VGLUT2 (K; red). The merged view is depicted in L indicating that the Chronos-GFP AAV transduced neurons associated with glutamatergic neurotransmission. M–P: a sec- ond representative spinal section transduced by Chronos-GFP AAV (M) and stained for NeuN (N), and GAD65 (O). The merged view is shown in P, indicating that the Chronos-GFP AAV also transduced neurons associated with GABAergic transmission. Q–T: a representative spinal section transduced by Chronos-GFP AAV (Q; green), NeuN (R; blue), and ChAT (S; red). The merged view is shown in T indicating that the Chronos vector also labels a small percentage of neurons associated with cholinergic transmission. Scale bar 20 m.
Polyclonal Rabbit Vesicular Glutamate Transporter 2 (Vglut2, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Fig. 3. Transduced neuronal cell types. Spinal sections were processed for immunoreactivity to NeuN, VGLUT2, GAD65, and ChAT to identify the neuronal cell types associated with the neurons transduced by ChR2-mCherry or Chronos-GFP adeno- associated viruses (AAV). White arrows highlight some of the positively stained GAD65, VGLUT2, and ChAT puncta that colocalized with transduced neurons. A–D: a representative spinal section transduced by ChR2-mCherry AAV (A; red) and stained for NeuN (B; blue), and VGLUT2 (C; green). D: merged view, indicating that the ChR2-mCherry AAV trans- duced neurons that are associated with glutamatergic neu- rotransmission. E–H: a second representative spinal section transduced by ChR2-mCherry AAV (E; red) stained for NeuN (F), and GAD65 (G). H: merged view showing that the ChR2- mCherry AAV transduced neurons associated with GABAergic transmission. I–L: a representative spinal section transduced by Chronos-GFP AAV (I; green) and stained for NeuN (J; blue), and VGLUT2 (K; red). The merged view is depicted in L indicating that the Chronos-GFP AAV transduced neurons associated with glutamatergic neurotransmission. M–P: a sec- ond representative spinal section transduced by Chronos-GFP AAV (M) and stained for NeuN (N), and GAD65 (O). The merged view is shown in P, indicating that the Chronos-GFP AAV also transduced neurons associated with GABAergic transmission. Q–T: a representative spinal section transduced by Chronos-GFP AAV (Q; green), NeuN (R; blue), and ChAT (S; red). The merged view is shown in T indicating that the Chronos vector also labels a small percentage of neurons associated with cholinergic transmission. Scale bar 20 m.

Journal: Journal of neurophysiology

Article Title: Optogenetic surface stimulation of the rat cervical spinal cord.

doi: 10.1152/jn.00461.2017

Figure Lengend Snippet: Fig. 3. Transduced neuronal cell types. Spinal sections were processed for immunoreactivity to NeuN, VGLUT2, GAD65, and ChAT to identify the neuronal cell types associated with the neurons transduced by ChR2-mCherry or Chronos-GFP adeno- associated viruses (AAV). White arrows highlight some of the positively stained GAD65, VGLUT2, and ChAT puncta that colocalized with transduced neurons. A–D: a representative spinal section transduced by ChR2-mCherry AAV (A; red) and stained for NeuN (B; blue), and VGLUT2 (C; green). D: merged view, indicating that the ChR2-mCherry AAV trans- duced neurons that are associated with glutamatergic neu- rotransmission. E–H: a second representative spinal section transduced by ChR2-mCherry AAV (E; red) stained for NeuN (F), and GAD65 (G). H: merged view showing that the ChR2- mCherry AAV transduced neurons associated with GABAergic transmission. I–L: a representative spinal section transduced by Chronos-GFP AAV (I; green) and stained for NeuN (J; blue), and VGLUT2 (K; red). The merged view is depicted in L indicating that the Chronos-GFP AAV transduced neurons associated with glutamatergic neurotransmission. M–P: a sec- ond representative spinal section transduced by Chronos-GFP AAV (M) and stained for NeuN (N), and GAD65 (O). The merged view is shown in P, indicating that the Chronos-GFP AAV also transduced neurons associated with GABAergic transmission. Q–T: a representative spinal section transduced by Chronos-GFP AAV (Q; green), NeuN (R; blue), and ChAT (S; red). The merged view is shown in T indicating that the Chronos vector also labels a small percentage of neurons associated with cholinergic transmission. Scale bar 20 m.

Article Snippet: Sections were washed with PBS 3 10 min at room temperature followed by blocking with 5% normal donkey serum (Sigma-Aldrich) in PBS for 1 h. Primary antibodies were then added to sections at the following dilutions: VGLUT2 (1:100; Origene Technologies, Rockville, MD; Zhang et al. 2018), GAD65 (1:500, Millipore, Billerica, MA; Besser et al. 2015), Synaptophysin (1:500, mouse host, Thermo Fisher Scientific, Waltham, MA; Haltas et al. 2012), Synaptophysin (1:250, rabbit host, Thermo Fisher Scientific; DeAndrade et al. 2012), NeuN (1:500, Millipore; for certificates of analysis see www.emdmillipore.com), ChAT (1:100; Millipore; Milan et al. 2015), and GFAP-Alexa Fluor 488 (1:500; Thermo Fisher Scientific; Xing et al. 2012).

Techniques: Staining, Transmission Assay, Plasmid Preparation